Thymogen is a dipeptide bioregulator (L-glutamyl L-tryptophan). It is used in lymphocyte and epithelial culture to study proliferation, differentiation markers and cytokine response, and NuVion stocks it in Australia as a 20mg lyophilised vial.
- Dipeptide bioregulator (L-glutamyl L-tryptophan)
- Used in lymphocyte and epithelial culture models
- Applied in proliferation, differentiation marker and cytokine assays
- Supplied lyophilised, purity by RP-HPLC and identity by mass spectrometry
Thymogen structure and research use
Thymogen is two amino acids long, glutamic acid joined to tryptophan, written EW in one-letter code. That makes it the shortest peptide in the range. It was first identified in thymic extract, the same source as the much longer Thymosin Alpha-1. In the laboratory it is used in lymphocyte and epithelial models for work on proliferation, differentiation markers and cytokine response.
Thymogen research applications
- qPCR in THP-1 monocytic cells measures TLR3, TLR7, TLR8, TLR9, RIG-I and MDA5 transcript levels
- ELISA quantifies TNF-α and IL-1β secretion alongside NF-κB reporter assays in monocytic and macrophage cell lines
- Endothelial cell proliferation, migration and tube-formation assays run with VEGF and FGF2 measured in conditioned medium
- Dipeptide uptake is measured in Caco-2 or PEPT1-transfected cells, including competition with Gly-Sar and pH dependence
Thymogen key facts
| Type | Synthetic dipeptide |
|---|---|
| Amino acid count | 2 |
| Sequence | L-Glu-L-Trp (alpha-linked) |
| Also known as | Oglufanide, Glu-Trp, EW dipeptide, glutamyl-tryptophan |
| Vial size | 20mg, lyophilised |
| Purity | 99.74% by RP-HPLC, Janoshik Analytical |
| Analysed | 28 October 2025 |
Research profile: Thymogen: structure, mechanism and research use
Independent test: a sample of this product was analysed by Janoshik Analytical on 28 October 2025, purity 99.74% by RP-HPLC. The certificate is shown in the lab band on this page.
