TB-500 is a synthetic peptide fragment derived from thymosin beta-4. It is used in actin binding and cell migration assays across fibroblast, endothelial and epithelial cultures, and NuVion stocks it in Australia as a 10mg lyophilised vial.
- Synthetic peptide fragment derived from thymosin beta-4
- Carries the Leu-Lys-Lys-Thr-Glu-Thr actin-binding motif
- Used in actin binding and cell migration assays
- Supplied lyophilised, purity by RP-HPLC and identity by mass spectrometry
TB-500 structure and research use
Thymosin beta-4 is a 43 amino acid protein and the main G-actin sequestering peptide in mammalian cells. TB-500 is a shorter synthetic fragment that carries the Leu-Lys-Lys-Thr-Glu-Thr motif through which thymosin beta-4 binds actin. In the laboratory it is used to study actin dynamics, cytoskeletal organisation and cell migration in fibroblast, endothelial and epithelial cultures.
TB-500 research applications
- Pyrene-actin polymerisation kinetics, with TB-500 as a low-affinity competitor of thymosin beta-4 and WH2 domains
- G-actin binding measured by isothermal titration calorimetry, fluorescence anisotropy or native gel shift
- Scratch and transwell migration assays in epithelial, fibroblast and endothelial cultures, read as closure of the cell-free gap
- F-actin imaging at the leading edge by phalloidin staining or live-cell actin reporters after peptide exposure
TB-500 key facts
| Type | Synthetic peptide, thymosin β4 fragment (residues 17 to 23) |
|---|---|
| Amino acid count | 7 |
| Sequence | Ac-Leu-Lys-Lys-Thr-Glu-Thr-Gln (Ac-LKKTETQ) |
| Also known as | Tβ4(17-23), thymosin β4 actin-binding fragment, TB500 |
| Vial size | 10mg, lyophilised |
| Purity | 99.91% by RP-HPLC, Janoshik Analytical |
| Analysed | 7 July 2026 |
Research profile: TB-500: structure, mechanism and research use
Independent test: a sample of this product was analysed by Janoshik Analytical on 7 July 2026, purity 99.91% by RP-HPLC, test number 195037. The full report opens from the lab band on this page.
