IGF-1 LR3 is a Long Arg3 analogue of insulin-like growth factor 1 with reduced affinity for IGF binding proteins, which extends its activity in culture. It is used in myoblast and fibroblast models to study protein synthesis and PI3K/Akt signalling, and NuVion stocks it in Australia as a 1mg lyophilised vial.
- Long Arg3 analogue of insulin-like growth factor 1
- Reduced affinity for IGF binding proteins, extending activity in culture
- Applied in protein synthesis and PI3K/Akt signalling assays
- Supplied lyophilised, purity by RP-HPLC and identity by mass spectrometry
IGF-1 LR3 structure and research use
IGF-1 LR3 is 83 amino acids long. It is the 70 amino acid IGF-1 sequence with arginine in place of glutamic acid at position 3 and a 13 amino acid extension on the N-terminus. Those two changes sharply reduce its binding to IGF binding proteins, so more of it stays free in culture medium. In the laboratory it is used in myoblast and fibroblast models for work on IGF-1 receptor signalling, protein synthesis and the PI3K/Akt pathway.
IGF-1 LR3 research applications
- Mitogenic additive in serum-free and chemically defined media for CHO and hybridoma production lines
- IGF-1R signalling studies reading phospho-Akt, phospho-S6K1 and phospho-ERK1/2 by western blot or in-cell assays
- BrdU or EdU incorporation and cell counting in fibroblast, epithelial and neuronal cell lines
- Ligand blot and surface plasmon resonance studies, as the low-affinity comparator for IGFBP binding
IGF-1 LR3 key facts
| Type | Recombinant IGF-1 analogue, IGF-1 receptor agonist |
|---|---|
| Amino acid count | 83 (13-residue extension plus the 70 residues of IGF-1) |
| Modifications | Glu3 to Arg substitution; N-terminal extension MFPAMPLSSLFVN; three disulfide bonds |
| Molecular weight | Approximately 9.1 kDa |
| CAS number | 946870-92-4 |
| Also known as | Long R3 IGF-1, LR3-IGF-I, Long Arg3 IGF-1, LR3IGF-1 |
| Vial size | 1mg, lyophilised |
| Purity | 99.87% by RP-HPLC, Janoshik Analytical |
| Analysed | 5 December 2025 |
Research profile: IGF-1 LR3: structure, mechanism and research use
Independent test: a sample of this product was analysed by Janoshik Analytical on 5 December 2025, purity 99.87% by RP-HPLC. The certificate is shown in the lab band on this page.
