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NuVion PE-22-28 research peptide vial

PE-22-28

A$69.00

PE-22-28 (Mini-Spadin), a selective TREK-1 channel blocker studied in neuroscience.

Research use only. Not for human or veterinary use.

In stock

All prices are in AUD. Ships from Australia with tracked express shipping, $15 flat, 1 to 2 business days, free on orders over $200. Research use only, not for human or veterinary use.

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Research use only

Not for human or veterinary use.

Laboratory chemical

Supplied for in vitro research. We publish specifications and handling guidance only, with no protocols and no advice on use.

Not on the ARTG

Absent from the Australian Register of Therapeutic Goods and never evaluated by the TGA for safety, quality or efficacy. Absence from the register is not an approval and does not authorise any other use.

Who can order

You must be 18 or over and purchasing solely for lawful research.

What is PE-22-28?

PE-22-28 is a synthetic seven amino acid analogue of spadin and a selective TREK-1 potassium channel blocker. It is used in neuronal culture and neurogenesis research models, with reported potency greater than the parent spadin sequence, and NuVion stocks it in Australia as a 10mg lyophilised vial.

  • Synthetic seven amino acid analogue of spadin (Gly-Val-Ser-Trp-Gly-Leu-Arg)
  • Selective TREK-1 potassium channel blocker
  • IC50 of 0.12 nM, more potent than parent spadin
  • Supplied lyophilised, purity by RP-HPLC and identity by mass spectrometry

PE-22-28 structure and research use

PE-22-28 is the short form of spadin, a peptide derived from the propeptide of the receptor sortilin. It keeps seven residues, Gly-Val-Ser-Trp-Gly-Leu-Arg, and is also known as Mini-Spadin. It blocks the TREK-1 potassium channel with a reported IC50 of 0.12 nM. In the laboratory it is used in neuronal culture and TREK-1 channel research models.

PE-22-28 research applications

  • Whole-cell and single-channel patch clamp on TREK-1 expressing cell lines, measuring block of the background potassium current
  • Selectivity profiling against TREK-2, TRAAK and other potassium conductances under matched recording conditions
  • Resting membrane potential and excitability measurement in primary neuronal culture
  • Neural progenitor proliferation and differentiation assays with BrdU or EdU incorporation and lineage marker staining

PE-22-28 key facts

Type Synthetic heptapeptide, spadin fragment
Amino acid count 7
Sequence H-Gly-Val-Ser-Trp-Gly-Leu-Arg-OH
Parent peptide Spadin, residues 22 to 28
Parent protein Sortilin propeptide
Molecular target TREK-1 (KCNK2) two-pore domain potassium channel
Reported potency IC50 in the sub-nanomolar range
Vial size 10mg, lyophilised
Purity 99.86% by RP-HPLC, Janoshik Analytical
Analysed 20 October 2025

Research profile: PE-22-28: structure, mechanism and research use

Independent test: a sample of this product was analysed by Janoshik Analytical on 20 October 2025, purity 99.86% by RP-HPLC. The certificate is shown in the lab band on this page.

Why buy PE-22-28 from NuVion

Testing status on every page

Published results show the measured purity, the date and the certificate.

Australian stock, domestic post

Held in Australia and sent tracked in plain outer packaging.

Specifications

Storing and handling PE-22-28

Before reconstitution
Keep the sealed vial cool, dry and out of direct light. General practice for lyophilised peptides.
After reconstitution
Reconstitute with bacteriostatic or sterile water, store at 2 to 8°C and avoid repeated freeze-thaw cycles. No stability-tested shelf life is published for this product.

Reconstitution and storage Volumes, concentrations and storage at a glance.

PE-22-28 questions

Where can I buy PE-22-28 in Australia?
NuVion stocks PE-22-28 in Australia as a 10mg vial of lyophilised powder, posted from Australian stock on a tracked express service. A sample was analysed by Janoshik Analytical on 20 October 2025, with purity of 99.86% by RP-HPLC. Bacteriostatic water is sold separately. PE-22-28 is supplied as a research material only and is not on the Australian Register of Therapeutic Goods.
What is PE-22-28 used for in research?
It is used as a selective pharmacological blocker of TREK-1 in patch clamp electrophysiology, in resting membrane potential and excitability measurement on neuronal culture, and in neural progenitor proliferation and differentiation assays where TREK-1 activity is the variable under test.
How does PE-22-28 work at the cell level?
PE-22-28 blocks TREK-1, a two-pore domain potassium channel encoded by KCNK2 that produces a background leak current setting the resting membrane potential of many neurons. The PE-22-28 research profile covers the mechanism in full.
How selective is PE-22-28 among potassium channels?
The block is described as selective for TREK-1 over the related two-pore channels TRAAK and TREK-2 and over the classical voltage-gated potassium currents. That selectivity is the property that makes it useful as a pharmacological tool.
Why is PE-22-28 handled in low-binding tubes?
Reported potency is in the sub-nanomolar range, so working dilutions are very dilute. Adsorption to plastic at these concentrations can account for a large fraction of the nominal amount, and serial dilutions are prepared in low-binding tubes with a carrier protein where the assay permits.
Which residue governs detection and stability of PE-22-28?
The tryptophan at position four. It provides absorbance at 280 nm and fluorescence near 350 nm for HPLC detection, and it is the residue most susceptible to oxidation and photodegradation, so solutions are kept out of the light.
Is PE-22-28 the same as Mini-Spadin?
Yes. Mini-Spadin is another name for PE-22-28. Both refer to the seven amino acid sequence Gly-Val-Ser-Trp-Gly-Leu-Arg.
What is the difference between PE-22-28 and spadin?
Spadin is the longer parent peptide. PE-22-28 is a shortened analogue of it with a reported IC50 of 0.12 nM at the TREK-1 channel, lower than the parent sequence.

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