Free reconstitution tool
Enter how much peptide is in your vial and how much water you're adding. You'll see the strength of the mix straight away, and if you add an amount, exactly how much liquid to draw in millilitres and units.Enter your vial and water to see the strength, then exactly how much to draw.
It's printed on the vial label, in mg. 1 mg is 1,000 mcg.
That's 10,000 mcg.
In ml. Most people use between 1 and 3.
Optional. Leave it blank to see the strength only.
This calculator converts the numbers you enter. It never suggests an amount, and NuVion does not provide dosing guidance for any compound. Products are supplied for research use only.
Example mix
On a U-100 scale, 1 unit is 0.01 ml.
An example with 10 mg in 2 ml of water. Change the numbers to match your vial.
Add an amount in step 3 to see exactly how much to draw.
Assumes a U-100 scale, where 100 units equal 1 ml and one unit is 0.01 ml. The powder is treated as adding no volume, since a few mg of freeze-dried peptide takes up around 0.01 ml. Round to the nearest mark your measuring device shows.
How it works
Every answer comes from three small sums. Here they are for a 10 mg vial mixed with 2 ml of bacteriostatic water.
Divide the peptide in the vial by the water you add. The answer is the strength of the mix in mg per ml.
10 mg ÷ 2 ml = 5 mg per mlDivide the amount you want by the strength, with both in mcg. The answer is the volume of liquid in ml.
500 mcg ÷ 5,000 mcg per ml = 0.1 mlA U-100 scale has 100 units in every ml, so multiply the volume by 100 to get units.
0.1 ml × 100 = 10 unitsAn easy way to picture it. Think of the powder as a teaspoon of salt. Stir it into a small glass or a large jug and there is still one teaspoon of salt. The water only sets how spread out it is, and so how much liquid holds the amount you want.
Reference chart
The strength you get from common vial sizes and water volumes. Each cell shows mg per ml, then how much peptide is in one unit, or 0.01 ml, on a U-100 scale.
Swipe the table to see 3 ml and 5 ml
| Vial | 1 ml water | 2 ml water | 3 ml water | 5 ml water |
|---|---|---|---|---|
| 5 mg | 5 mg/ml50 mcg per unit | 2.5 mg/ml25 mcg per unit | 1.67 mg/ml16.7 mcg per unit | 1 mg/ml10 mcg per unit |
| 10 mg | 10 mg/ml100 mcg per unit | 5 mg/ml50 mcg per unit | 3.33 mg/ml33.3 mcg per unit | 2 mg/ml20 mcg per unit |
| 15 mg | 15 mg/ml150 mcg per unit | 7.5 mg/ml75 mcg per unit | 5 mg/ml50 mcg per unit | 3 mg/ml30 mcg per unit |
| 20 mg | 20 mg/ml200 mcg per unit | 10 mg/ml100 mcg per unit | 6.67 mg/ml66.7 mcg per unit | 4 mg/ml40 mcg per unit |
| 30 mg | 30 mg/ml300 mcg per unit | 15 mg/ml150 mcg per unit | 10 mg/ml100 mcg per unit | 6 mg/ml60 mcg per unit |
| 50 mg | 50 mg/ml500 mcg per unit | 25 mg/ml250 mcg per unit | 16.7 mg/ml167 mcg per unit | 10 mg/ml100 mcg per unit |
A 15 mg vial in 3 ml and a 5 mg vial in 1 ml both come out at 5 mg per ml. The strength depends on the ratio of powder to water, so different vials can make exactly the same mix.
Reference chart
Volumes for a range of amounts at 5 mg per ml, the strength you get from 10 mg in 2 ml. For any other mix, use the calculator above.
| Amount | In mg | Volume | U-100 units |
|---|---|---|---|
| 100 mcg | 0.1 mg | 0.02 ml | 2 units |
| 250 mcg | 0.25 mg | 0.05 ml | 5 units |
| 500 mcg | 0.5 mg | 0.10 ml | 10 units |
| 750 mcg | 0.75 mg | 0.15 ml | 15 units |
| 1,000 mcg | 1 mg | 0.20 ml | 20 units |
| 1,500 mcg | 1.5 mg | 0.30 ml | 30 units |
| 2,000 mcg | 2 mg | 0.40 ml | 40 units |
| 2,500 mcg | 2.5 mg | 0.50 ml | 50 units |
| 5,000 mcg | 5 mg | 1.00 ml | 100 units |
At 5 mg per ml, every 50 mcg is 1 unit. The general rule is units = amount in mcg ÷ strength in mcg per ml × 100.
Mixing
There is no single right amount. The peptide in the vial stays the same whatever volume of water you add. The water sets how much liquid that peptide is spread through, and the liquid is what you measure.
More water gives a weaker mix, so each amount takes up more liquid and is easier to measure. Less water gives a stronger mix and smaller volumes, which are harder to read accurately. Most people use 1 to 3 ml and pick the volume that lands their draw at a comfortable mark, often between 5 and 50 units.
Once the vial is mixed, that ratio is set until it is finished, so run the numbers in the calculator before you add the water.
Same 10 mg vial, same 500 mcg
| Water | Strength | 500 mcg is |
|---|---|---|
| 1 ml | 10 mg/ml | 0.05 ml, 5 units |
| 2 ml | 5 mg/ml | 0.10 ml, 10 units |
| 3 ml | 3.33 mg/ml | 0.15 ml, 15 units |
| 5 ml | 2 mg/ml | 0.25 ml, 25 units |
Method
Reconstituting means adding liquid to freeze-dried powder so it can be measured. Bacteriostatic water is sterile water with a small amount of benzyl alcohol, usually 0.9%, added as a preservative so the vial can be drawn from more than once. The standard lab method takes a couple of minutes.
FAQ
Divide the amount you want by the strength of the mix to get millilitres, then multiply by 100 to get units on a U-100 scale. A 10 mg vial mixed with 2 ml of water holds 5,000 mcg per ml, so 500 mcg is 0.1 ml, which is 10 units.
Any amount works for the maths, and most people use 1 to 3 ml. With 2 ml in a 10 mg vial the mix is 5 mg per ml, where every 50 mcg is 1 unit, which keeps the numbers easy to work with. Compare a few options in the chart above before you mix.
One mg is 1,000 mcg. Vials are labelled in mg while amounts are often written in mcg, and mixing the two up is the most common mistake people make. The calculator accepts the amount in either unit and shows the conversion under each box.
It is the strength of the mix: how many milligrams of peptide are in each millilitre of liquid. At 5 mg per ml, 1 ml holds 5 mg and 0.1 ml holds 0.5 mg, which is 500 mcg.
U-100 means 100 units in every ml, so one unit is always 0.01 ml. The common 0.3 ml, 0.5 ml and 1 ml insulin syringes all use this scale. They differ in capacity and in how finely they are marked: 0.3 ml and 0.5 ml barrels usually have a line for every unit, some 0.3 ml brands mark every half unit, and 1 ml barrels usually mark every 2 units.
It does the same maths. You enter an amount and it converts that amount into a volume in ml and units. NuVion does not recommend amounts for any compound, and nothing on this page suggests one.
Divide the total in the vial by the amount in each draw. A 10 mg vial holds 10,000 mcg, so at 500 mcg a draw there are 20 draws. The water you add has no effect on this number.
Barely. A few milligrams of freeze-dried peptide takes up around 0.01 ml, well under 1% of a 2 ml mix, so the calculator treats the water you add as the final volume.
Your mix is strong, so a small volume holds a lot of peptide. Anything under 2 units, or 0.02 ml, is hard to measure accurately. Using more water when you mix the next vial spreads the same amount through more liquid and gives a bigger volume that is easier to read.
You can, although every number changes from that point and you would need to recalculate using the new total volume. Choosing the ratio before you mix is simpler, which is what the calculator is for.
In the fridge, upright and away from light. General lab guidance treats the fridge as short-term storage for peptides in solution, around a week, and freezing single-use portions as the longer-term option. Avoid freezing and thawing the same vial repeatedly. Stability varies from compound to compound, so write the date on the label when you mix it.
Keep reading
Need bacteriostatic water?Dispatched from Australia with your order.
Shop BAC waterLast reviewed 29 September 2026. The charts and worked examples on this page were checked against the calculator.
NuVion Health supplies research peptides for laboratory research use. This calculator converts the numbers you enter and does not recommend an amount for any compound. Always confirm your figures against the vial label before you mix.
